Anti-β-Catenin Antibody (M118)

3 Citations

Our β-Catenin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat β-Catenin and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).
Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).
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Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).
Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-β-Catenin (CM1181) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse

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SKU: CM1181

Volume: 100 µL
1-2 business days
Price:
$370.00

Product Details

β-Catenin
β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Protein A Purified
Monoclonal
M118
IgG1
ICC, IHC, IP, WB
Mouse
CTNNB1
92
Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
IHC: 1:500
ICC: 1:250
IP: 1:100
Unconjugated
The antibody detects a 92 kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only β-Catenin in immunoprecipitations using anti-β-Catenin versus γ-Catenin.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Catenin beta1, CTNNB1, catenin
P35222

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
23911227not listedGayrard, C, et al. 2018. Src- and confinement-dependent FAK activation causes E-cadherin relaxation and β-catenin activity. The Journal of cell biology, 1063-1077.
Immunocytochemistry: Mouse
PMID Dilution Publication
332072241:250Muncie, JM, et al. 2020. Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem Cells. Developmental cell, 679-694.
Western Blot: Human
PMID Dilution Publication
29311227not listedGayrard, C, et al. 2018. Src- and confinement-dependent FAK activation causes E-cadherin relaxation and β-catenin activity. The Journal of cell biology, 1063-1077.

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